Coding

Part:BBa_K2020052:Design

Designed by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher   Group: iGEM16_Aachen   (2016-10-15)


DMNBS-Synthetase for use in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 84
    Illegal SapI.rc site found at 877


Design Notes

The synthetase was created via mutation library following computational design from Methanococcus janaschii wild type tyrosyl synthetase.


Source

Basis for mutation was Methanococcus janaschii wild type tyrosyl synthetase.

References

  1. E. A. Lemke, D. Summerer, B. H. Geierstanger, S. M. Brittain, and P. G. Schultz, “Control of protein phosphorylation with a genetically encoded photocaged amino acid,” Nat. Chem. Biol., vol. 3, no. 12, pp. 769–772, Dec. 2007.